A Review On Plant Physiology Mini Project : Investigating The Antibacterial Activity of Phyllanthus amarus Against Escherichia coli

 During my second year, my group and i were assigned to conduct an experiment regarding herbal plants under Plant Physiology subject. We chose to study the effect of Phyllanthus amarus againts the activity of Escherichia coli.

Phyllanthus amarus (Dukung anak)

We performed our investigation and experiments along eleven weeks given. And during the 13th week, our finding was presented during the symposium which was held on 22nd of September 2010. Here are bits of what we have summarized, discussed and concluded.

The antibacterial activity of the extracts of the root, leaf and stem of Phyllanthus amarus was assessed against Escherichia coli. The extraction process was done by grinding 5 grams of parts of root, leaf and stem respectively of the fresh plant in a clean mortar and blender. The extract was filtered afterwards. The method that we used to carry this experiment is by using disc diffusion method. Using Stokes Disc diffusion sensitivity testing technique, an inoculums containing bacterial or yeast cells was applied onto nutrient agar plates.

We collected our specimens from alleys and beside drains in front of shops in Taman Bahtera, Hulu Bernam

After the extraction process, we cultured E.coli .

We used the Disk Diffusion techniques.

The results of this investigation showed that plant extracts of Phyllanthus amarus does posses potential antibacterial activity against commonly gastro enteric microorganism, E.coli which affects the intestinal epithelial cells producing enter toxins that leads to secretion of electrolytes and water into the lumen of the small intestine, manifested as watery diarrhea. Hence, this study more or less has revealed and proved that this plant can be adapted for prevention intestinal related diseases which are caused by invasion of E.coli.

For more details regarding our report please refer to this link below :
Investigating The Antibacterial Activity Of Phyllanthus amarus against Escherichia coli